FIGURE 1 from Exercise-induced Β2-Adrenergic Receptor Activation Enhances the Antileukemic Activity of Expanded Γδ T-Cells Via DNAM-1 Upregulation and PVR/Nectin-2 Recognition

Exercise preferentially mobilizes γδ T-cells with phenotypic and transcriptomic profiles associated with anti-tumor immunity. A, The percentage or MFI of activating, inhibitory, and chemokine receptors among Vδ2+ and Vδ1+ T-cells before (REST) and during (EX) acute exercise (n = 8). Significance is indicated by * (P < 0.05). B, GSEA performed using KEGG and GO terms showing enriched pathways on exercise mobilized γδ T-cells (n = 3). Graphs show enriched upregulated (blue) and downregulated (red) pathways as well as the percentage of DEGs contributing to each mechanism. Statistical significance set as FDR ≤ 0.25. C, Azimuth map for human PBMCs showing the 26 clusters identified by scRNA-seq at rest; γδ T-cells mapping is circled in red. (n = 3). D, Chord diagram displaying the leading-edge genes driving the enrichment of terms associated with cytotoxicity and anti-tumor signaling in γδ T-cells (n = 3). Data are represented as mean; *, P < 0.05, Students two-tailed paired t test (A); * ≤0.25, FDR (B).