Muscle fibre size and myonuclear positioning in trained and aged humans

Abstract Myonuclear domain (MND) is the theoretical volume of cytoplasm within which a myonucleus is responsible for transcribing DNA. Changes in myonuclear number, organisation, and myonuclear domain size are associated with exercise adaptations and ageing. However, data on satellite cell activation, changes in MND volumes and myonuclear arrangement following exercise are inconsistent. Additionally, whether MNDs and myonuclear arrangement are altered with age remains unclear. The aim of the present investigation was therefore to investigate relationships between age and activity status and myonuclear numbers and organisation. Muscle fibres from younger trained (YT) and older trained (OT) individuals were compared with age-matched untrained counterparts (YU and OU). Serial, optical z-slices were acquired throughout isolated muscle fibres and analysed to give 3D coordinates for myonuclei and muscle fibre dimensions, respectively. As expected, mean cross-sectional area (CSA) (μm 2 ) of muscle fibres from OU was 29-42% smaller compared to the other groups. Number of nuclei relative to fibre CSA was 87% greater in OU compared to YU muscle fibres (P < 0.05). Additionally, scaling of myonuclear domain volume with fibre size was altered in older untrained individuals. Myonuclear arrangement, on the other hand, was similar across groups. These data indicate that regular endurance exercise throughout the lifespan may preserve the size of single muscle fibres in older age and maintain the relationship between fibre size and MND volumes. Inactivity, however, may result in reduced muscle fibre size and disrupted relationship between fibre size and MND volumes. Plain Language Summary In this study, we examined the relationship between physical activity and the characteristics of muscle fibres in individuals of different age groups. We focused on a concept called the myonuclear domain (MND), which refers to the volume surrounding muscle nuclei or myonuclei that house the genome. We wanted to understand how changes in myonuclear number, organisation, and MND size were influenced by exercise and aging. To do this, we compared muscle fibres from younger trained individuals, older trained individuals, and age-matched untrained individuals. The results showed that the average size of muscle fibres in the untrained older individuals was smaller compared to the other groups. Moreover, the number of nuclei relative to fibre size was significantly higher in the untrained older individuals. However, myonuclear arrangement was similar across all groups. These findings suggest that regular endurance exercise throughout life may help maintain muscle fibre size, myonuclear numbers, MND volumes, and myonuclear organisation in older individuals. Conversely, inactivity can lead to reduced muscle fibre size and disrupted relationship between fibre size and MND volumes. These results have important implications for understanding the effects of exercise and aging on muscle health.