ImmunoInertial Microfluidics: A Novel Strategy for Isolation of Small EV Subpopulations

•small extracellular vesicles (sEVs) are highly heterogeneous, posing technical challenges to acquiring their molecular information.•The synergic effects of the immunoaffinity-based approach and inertial microfluidics (ImmunoInertial microfluidics) were proposed to isolate specific subpopulations of sEVs.•In the first step, the cancer cell-derived sEVs were captured on microbeads of varying sizes which were functionalized with specific capture antibodies.•Then, the sEVs-bearing microbeads were subsequently introduced to a series of inertial microfluidic channels called iZExoSub (inertial zigzag microfluidics for exosome subpopulation separation) for size-based bead separation.