Protein Profiling of Guillain–Barrè Syndrome Cerebrospinal Fluid by Two-Dimensional Electrophoresis and Mass Spectrometry

Simona D'Aguanno,Diego Franciotta,Santina Lupisella,Alessandra Barassi,Damiana Pieragostino,Alessandra Lugaresi,Diego Centonze,Gianlodovico Melzi D'Eril,Sergio Bernardini,Giorgio Federici,Andrea Urbani

Neuroscience Letters（2010）SCI 4区

Univ Roma Tor Vergata | IRCCS | Univ Milan | Ctr Studi Sull Invecchiamento CeSI | IRCCS Fdn Santa Lucia

Cited 29|Views13

Abstract

Protein profiling of cerebrospinal fluid in Guillain-Barrè syndrome (GBS), an acute and immune-mediated disease affecting the peripheral nervous system, was performed by two-dimensional electrophoresis. Significant modulated spots in GBS patients vs. control groups (a group of multiple sclerosis patients and one of healthy donors) underwent MALDI-TOF/TOF investigation. Inflammation-related proteins, such as vitamin D-binding protein, beta-2 glycoprotein I (ApoH), and a complement component C3 isoform were up-regulated in GBS, whereas transthyretin (the monomer and the dimer forms), apolipoprotein E, albumin and five of its fragments were down-regulated. Then, we used an isoelectric-focusing-dinitrophenylhydrazine-based technique to analyse the extent of carbonylation and, as a result, of oxidative damage of GBS CSF proteome. We observed a major sensitivity to carbonylation for albumin and alpha-glycoprotein in inflammation and a selective increase of reactivity for a glycosylated Fab from an IgM globulin in GBS CSF. Our results add new proteins to candidate CSF features of GBS, and suggest that oxidative stress could contribute to the immunopathological mechanisms in this syndrome.

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Key words

Guillain-Barre syndrome,CSF,Proteomics,Biomarkers,Oxidative damage,Protein carbonylation

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