Meregalli Mirella

Personal data Name: Mirella Meregalli Birthdate: 20/11/1971 Birthplace: Milano Address: Via F. Sforza, 35 20122 Milan Italy Telephone: 0039-02-55030374 Fax: 0039-02-50320430 e-mail: mirella.meregalli@unimi.it Education and training 1998 DEGREE IN BIOLOGY, University of Milan TITLE OF TESIS: “Effect of somatostatin and BIM-23014 in human cancer cells line “ 2003 Resident in Toxicology, University of Milan Project: Hemochromatosis and iron overload, genetic and molecular characterization Employment and research experience · February 1996/ May 1998 Student in Department of “Pharmacology” of Biology Faculty , University of Milan, under the supervision of Prof. Lucia Vicentini. Project: Evaluation of the effects of somatostatin on proliferation of human cancer cells line - preparation of different human cancer cell lines; - extraction of proteins from cells and tissues - SDS-page and western blot analysis of growth factors receptors and intracellular signal proteins - preparation of cells culture and treatments with different drugs - extraction of RNA and DNA - RT-PCR analysis - Preparation of bacteria and extraction of recombinant proteins · September 1998/January 2003 Resident in toxicology in Department of Internal Medicine, Fondazione IRCCS Policlinico di Milano under the supervision of Dott M. Sampietro Project: Hemochromatosis and iron overload, genetic and molecular characterization Techniques: - DNA extraction from cells, serum and tissues - Restriction analysis of genomic DNA e of PCR products - Elettroforetics tecniques (SSCP, heteroduplex) for mutation screening - Cloning of PCR products - Direct sequencing of PCR products - Binding tecniques · February 2003/ present PhD: research activity in “Stem Cell Laboratory” under the supervision of Dott. Torrente. Project: characterization of murine and human stem cells from muscle, blood and neural origin Techniques: - Immunostaining analysis of muscle cryosections - Laser dissection : single cells isolation from muscle and brain and RNA extraction - Realtime PCR