1. Bioluminescence and fluorescence tomography-guided radiation therapy for pre-clinical study Goal: Advancing highly-accurate radiation delivery and quantitative 3D in vivo optical imaging for pre-clinical study 2. Validate linear energy transfer (LET) dosimetry for proton therapy using 3D optical imaging and high precision proton-small animal radiation system Goal: Understand proton dosimetry to resolve proton cancer therapy uncertainities in patient treatment