The Lilley Group focuses on the development and application of technologies which enable the measurement of the dynamic proteome in space and time in cells in a high-throughput manner. More specifically, we are interested in looking at the changes in abundance, location, interacting partners, post-translational modification status and structure of proteins during dynamic cellular processes such as differentiation. We also develop methods to determine the subcellular distribution of the transcriptome and how this changes upon perturbation, and the complement of proteins that form dynamic ribonuclear complexes. Finally, we aim to determine the spatial and temporal links between the trafficking of the proteome and its corresponding transcriptome.

Our technologies involve innovative experimental pipelines coupled with quantitative mass spectrometry, RNA-seq, and computational tools. We use these methods to dissect cellular mechanisms related to stress, drug treatment, differentiation and disease in many collaborative projects with academic labs and the pharmaceutical industry.