I have been engaged in biomedical research for nearly 30 years with a focus on bacterial infections of the airways in persons with cystic fibrosis. My laboratory has developed novel methods of bacterial genotyping to study the molecular epidemiology of CF related respiratory tract pathogens, as well as numerous genotype-based methods for bacterial species identification. Previous work related to bacterial pathogenesis utilized cell culture and animal models of infection. My laboratory serves as a national reference laboratory for the Cystic Fibrosis Foundation, and maintains an extensive culture collection that includes approximately 35,000 strains of respiratory tract bacterial pathogens, including Burkholderia, Pseudomonas, Stenotrophomonas, Achromobacter, Haemophilus, Acinetobacter, and Staphylococcus spp. During the past 5 years, research in my laboratory has focused on culture-independent microbial community profiling, including use of microbiomic, transcriptomic, and metabolomic platforms, to understand the lung microbiology in CF.