Proteins exert their functions in our cells and tissues because they adopt a defined three dimensional structure. Stressful conditions, such as increased temperature or oxidative stress, can cause protein unfolding and thus disturb the integrity of the proteome. As a protective measure cells employ molecular chaperones, which recognize unfolded proteins, prevent their aggregation and promote folding to the functional conformation. In case of terminal damage, chaperones cooperate with cellular degradation systems to facilite protein disposal. We are studying molecular mechanisms essential for proteome maintenance and elucidate its regulation in diseases.