We study the biology of immune cells and their roles in the pathogenesis of cancers and other life-threatening diseases. By applying new and more precise analytical tools for assessing this system in mice and humans, we have been successful at identifying disease-promoting immune abnormalities. By targeting the cells responsible for or affected by these abnormalities, we have succeeded in reversing the abnormalities and ameliorating the diseases they cause. We make extensive use of mouse models for in depth mechanistic studies, but we also use human tissues to confirm the clinical relevance of our findings. To pursue this work, we have been using newer multiplex imaging technologies and deep gene sequencing.