Development and characterization of active packaging system based on zein nanofibers mat incorporated with geraniol-loaded nanoliposomes

In recent years, development of biopolymeric nanofibers as an active biodegradable packaging system has attracted specific attention. The objective of this research was to develop zein-based electrospun nanofibers (NFs) incorporated with geraniol-loaded nanoliposomes (G-loaded NLPs). Geraniol was encapsulated into NLPs with an efficiency of 79.23%. The particle size and zeta potential of G-loaded NLPs were 121.50 nm and -38.30 mV, respectively. The successful loading of geraniol in the NLPs was approved by Fourier transform infrared (FT-IR) spectroscopy. The liposomal vesicles showed spherical shapes. G-loaded NLPs were added in the zein-based electrospun NFs at three different concentrations (0.25, 0.5, and 1%w/v). All NFs samples exhibited fibrillar structure. The increase of NLPs concentration enhanced the thermal stability of the NFs. However, the crystalline structure of zein NFs did not change by the addition of G-loaded NLPs. The highest surface hydrophobicity was related to the NFs containing 1% G-loaded NLPs. The mechanical parameters of NFs depend on the concentration of NLPs. The NFs incorporated with G-loaded NLPs showed inhibition activity against four foodborne pathogenic bacteria (Staphylococcus aureus, Listeria monocytogenes, Escherichia coli, and Salmonella typhimurium) with an inhibition zone of 4.5-22 mm. Moreover, the alpha-diphenyl-beta-picrylhydrazyl (DPPH) scavenging activity of NFs samples was located at the range of 20%-48%. These findings represent the Efficiency of the G-loaded NLPs for use as bioactive compound in the zein-based NFs as an active packaging material. Geraniol (G) was successfully encapsulated in nanoliposome (NLPs) carriers and the active zein electrospun nanofibers were successfully reinforced with NPLs. All nanofibers were fibrillary in shape with relative crystalline nature and the G-loaded NLPs created strong antioxidant/antimicrobial activity in fibers. The fabricated nanofibers are suggested as promising food active packaging.image