A Simplified and Robust Model for the Study of Diabetic Nephropathy: Streptozotocin-Induced Diabetic Mice Fed a High-Protein Diet

Océane Pointeau, Romain Barbosa, Maéva Loriot,Julia Leemput, Elisabeth Dubus, Sébastien Zwe Causse,Laurent Demizieux, Patricia Passilly-Degrace,Pascal Degrace,Bruno Vergès,Tony Jourdan

International journal of molecular sciences（2025）

UFR Sciences Vie Terre Environnement | ImaFlow Core Facility

Cited 0|Views1

Abstract

To better understand diabetic nephropathy (DN), developing accurate animal models is crucial. Current models often fail to fully mimic human DN, showing only mild albuminuria, glomerular hypertrophy, and limited mesangial matrix expansion. Our study aims to develop a more robust model by combining streptozotocin (STZ)-induced diabetes with a high-protein diet (HPD). We divided C57Bl/6J mice into three groups: control, STZ with a standard diet (STZ-SD), and STZ with a HPD (45 kcal% protein) (STZ-HPD) for 12 weeks. Renal function was evaluated using the urinary albumin-to-creatinine ratio, and kidney tissues were analyzed for histological and molecular changes. The STZ-HPD group showed significantly higher albuminuria and more severe glomerular and tubular damage compared to the control and STZ-SD groups. These changes were accompanied by increased inflammatory and oxidative stress markers, highlighting the harmful effects of high-protein intake on renal injury. Our findings suggest that the STZ-HPD model could be a valuable tool for studying DN pathophysiology and evaluating therapeutic interventions, providing a new approach for preclinical research.

Translated text

Key words

C57Bl/6J mice,high protein diet,streptozotocin,diabetic nephropathy

求助PDF

上传PDF

Bibtex

AI Read Science

AI Summary

AI Summary is the key point extracted automatically understanding the full text of the paper, including the background, methods, results, conclusions, icons and other key content, so that you can get the outline of the paper at a glance.

Example

Background

Key content

Introduction

Methods

Results

Related work

Fund

Key content

Pretraining has recently greatly promoted the development of natural language processing (NLP)
We show that M6 outperforms the baselines in multimodal downstream tasks, and the large M6 with 10 parameters can reach a better performance
We propose a method called M6 that is able to process information of multiple modalities and perform both single-modal and cross-modal understanding and generation
The model is scaled to large model with 10 billion parameters with sophisticated deployment, and the 10 -parameter M6-large is the largest pretrained model in Chinese
Experimental results show that our proposed M6 outperforms the baseline in a number of downstream tasks concerning both single modality and multiple modalities We will continue the pretraining of extremely large models by increasing data to explore the limit of its performance

Upload PDF to Generate Summary

Must-Reading Tree

Example

Generate MRT to find the research sequence of this paper

Data Disclaimer

The page data are from open Internet sources, cooperative publishers and automatic analysis results through AI technology. We do not make any commitments and guarantees for the validity, accuracy, correctness, reliability, completeness and timeliness of the page data. If you have any questions, please contact us by email: report@aminer.cn

Chat Paper

【要点】：本研究开发了一种新的糖尿病肾病（DN）动物模型，通过结合链脲霉素（STZ）诱导的糖尿病和高蛋白饮食（HPD）来更好地模拟人类DN的病理特征。

【方法】：研究将C57Bl/6J小鼠分为三组，分别接受对照饮食、STZ加标准饮食（STZ-SD）和STZ加高蛋白饮食（STZ-HPD）处理，持续12周，通过尿蛋白肌酐比值评估肾功能，并对肾脏组织进行组织学和分子学分析。

【实验】：实验使用C57Bl/6J小鼠，通过STZ诱导糖尿病并结合高蛋白饮食（STZ-HPD）处理12周，发现STZ-HPD组相比对照组和STZ-SD组显示出更严重的蛋白尿和肾小球及肾小管损伤，伴随着炎症和氧化应激标志物的增加，表明该模型在研究DN病理生理学和评估治疗方法方面具有潜在价值。