Silver Nanostructures for Determination of FKBP12 Protein

Cosimo Bartolini, Martina Tozzetti,Stefano Menichetti,Gabriella Caminati

Engineering Proceedings（2024）

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Abstract

FKBP12 is a peptidyl––prolyl cis–trans isomerase that was recently proposed as a candidate biomarker for cancer, for neurodegenerations and for anti–rejection therapy after organ transplant. We designed and fabricated a nanosensor platform for the rapid and efficient determination of FKBP12 concentration in biological fluids exploiting anisotropic silver nanoparticles (AgNps) to enhance the capabilities of Quartz Crystal Microbalance (QCM) detection. To this end, the QCM sensor was coated with a compact array of AgNPs that were further functionalized with a Self–Assembled–Monolayer containing a synthetic receptor, GPS–SH1, designed and synthesized specifically to selectively bind FKBP12. Silver nanoflowers, AgNFs, and silver dendrites, AgNDs, were prepared by electrodeposition and characterized by means of UV–Vis spectroscopy, Scanning Electron Microscopy (SEM), QCM and water contact angle (CA). The AgNPs@Au/GPS–SH1–functionalized QCM sensors were used to detect increasing concentrations of FKBP12 in solution; the results showed that the use of AgNDs significantly enhanced the sensitivity of the sensor with respect to flat Ag sensor chips, allowing the detection of FKBP12 at sub–picomolar concentrations.

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Key words

FKBP12,nanosensors,silver nanostructures,neurodegenerative diseases,cancer,transplant rejection

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【要点】：本研究设计并构建了一种基于石英晶体微平衡（QCM）技术的纳米传感器平台，利用各向异性银纳米颗粒（AgNPs）提高FKBP12蛋白质的快速检测效率，实现了亚皮摩尔级别的FKBP12浓度检测。

【方法】：通过在QCM传感器表面涂覆紧凑的AgNPs阵列，并进一步与能选择性结合FKBP12的合成受体GPS-SH1自组装单分子层功能化。

【实验】：制备了银纳米花（AgNFs）和银树枝状结构（AgNDs）并通过电极沉积方法进行表征，使用UV-Vis光谱、扫描电子显微镜（SEM）、QCM和水接触角（CA）技术。实验中，AgNPs@Au/GPS-SH1功能化的QCM传感器用于检测溶液中FKBP12的浓度，结果显示使用AgNDs显著提高了传感器的灵敏度，允许在亚皮摩尔浓度水平上检测FKBP12。