Tree Species Identity Affects Soil P Bioavailability by Altering Labile Organic P after Tree Mixing in Subtropical China

Converting monocultures to mixed plantations has been emphasized to improve ecosystem productivity and services. However, the impact of tree species identity on phosphorus (P) bioavailability in acidic soils in subtropical China, where P is relatively scarce, is not fully understood. This study explored the changes in soil biologically-based P fractions and the effect of mineral and microbial properties on P transformation after mixing five broadleaved trees (Bretschneidera sinensis, Manglietia conifera, Cercidiphyllum japonicum, Michelia maudiae and Camellia oleifera) individually with coniferous trees (Pinus massoniana). The results showed that most mixed plantations significantly increased pH and citric acid and decreased exchangeable Fe3+ and Al3+ and the activation of Fe and Al oxides compared with monospecific plantations, which significantly reduced P precipitation and adsorption. Mixed planting significantly increased phosphatase activity and altered the community composition of P-mobilizing bacteria carrying phoD and pqqC genes, which contributed to organic P mineralization and inorganic P (Pi) desorption. Mixed planting increased microbial biomass and the relative rate of microbial biomass P turnover. Labile organic P (Enzyme-P) was a potentially significant source of soluble Pi (CaCl2-P) among the biologically-based P fractions, plus microbial biomass P. Overall, introducing broadleaved species, especially in species (e.g. Cercidiphyllum japonicum, Michelia maudiae and Camellia oleifera) with relatively high litter quality and belowground secretions (e.g. citric acid, phosphatase), significantly increased the solubilization of recalcitrant Pi (HCl-P), desorption of chemisorbed Pi (Citrate-P) and accumulation and mineralization of Enzyme-P, thereby increasing the available P pools. Redundancy analysis demonstrated that P fractions were mainly driven by phosphatases, exchangeable cations, floor fresh litter lignin/N and citric acid. Altogether, we highlight the importance of choosing tree species mixtures that have synergistic or complementary effects when constructing mixed plantations in order to alleviate soil P limitations. Responses and mechanistic regulation of soil phosphorus (P) bioavailability and transformation by introducing broadleaved species into monocultural coniferous plantations.image