Supplementary Figure S4 from Inhibition of Notch4 Using Novel Neutralizing Antibodies Reduces Tumor Growth in Murine Cancer Models by Targeting the Tumor Endothelium

A-B, Growth curves of subcutaneous B16F10 (n=3) and orthotopic Py8119 tumors (n=3) implanted into C57BL/6 mice and treated twice weekly IV with 25 m/kg of E7011 until tumors reached roughly 200mm3. Treatment duration was 4 days for B16F10 and 10 days for Py8119. C, Dose response curve of Calu-6 xenografts implanted subcutaneously in nude mice and treated with either control IgG, 1mg/kg, 3mg/kg or 10mg/kg of E7011 IV for 8 days (n=8). D, Representative images of CD31 staining and Hoescht dye staining following treatment. E, Quantitation of Hoescht+ stained area over total area. All quantitation were normalized to the control IgG. Statistics by 2-way ANOVA for growth curves and t-test for perfusion studies; *p<0.05, **p<0.01, ***p<0.001.