Mechanism of Lacticaseibacillus Rhamnosus JY027 Alleviating Hyperuricemia in Mice Through Gut-Kidney Axis

Hyperuricemia is associated with various diseases. This study confirmed that intervention with Lacticaseibacillus rhamnosus JY027 (L. rhamnosus JY027) reduced biochemical markers including uric acid, creatinine, and urea nitrogen in hyperuricemic mice. A combined analysis of gut microbiota, short-chain fatty acids (SCFAs), and metabolites based on the gut-kidney axis revealed that this bacterial intervention significantly enhanced the abundance of beneficial bacteria such as Bifidobacterium, Akkermansia, and Faecalibaculum and increased SCFA levels. The bacterial intervention also regulated the levels of guanosine, creatinine, arginine, choline, taurine and other differential metabolites in the kidneys of mice with hyperuricemia. This intervention ultimately reduced uric acid levels in mice through the modulation of multiple pathways, including arginine biosynthesis, taurine and hypotaurine metabolism, and glutathione metabolism. This study lays the foundation for exploring how L. rhamnosus JY027 alleviates hyperuricemia, providing theoretical support for developing probiotic preparations to mitigate hyperuricemia and their application.