AC Magnetic Field-Driven Wireless Charging Dual-Oriented Fibrous Magnetoelectric Scaffold CFO/PVDF Promotes Peripheral Nerve Repair

Efficient repair of peripheral nerve injuries (PNI) poses a significant challenge in neurosurgery. Wireless charging systems developed from piezoelectric materials, which can convert various forms of exogenous or endogenous mechanical signal into electrical stimulation, are highly regarded in the field of nerve regeneration. However, the potential mobility restrictions of patients with PNI and the attenuation of mechanical signal in deep tissues make it difficult to generate optimal electrical stimulation for inducing nerve regeneration, thereby limiting their clinical applications. Here, we developed a dual-oriented fibrous scaffold with excellent magneto-electric conversion performance to provide stable and controllable wireless electrical stimulation for PNI repair. The oriented arrangement of cobalt ferrite (CoFe2O4, 2 O 4 , CFO) fibrous fillers combined with the aligned orientation of polyvinylidene fluoride (PVDF) fibers improves the magneto-electric conversion performance of the scaffolds, resulting in the generation of approximately 150 mV under a low-frequency alternating magnetic field (150 Oe, 1000 Hz). Gait analysis demonstrated that stable electrical stimulation resulted in significant motor function recovery in rats when using the scaffold wrapped around Gelatin Methacryloyl (GelMA) hydrogel with neural stem cells (NSCs) under an AC magnetic field. Further, single-cell transcriptomics and histological studies confirmed that the scaffold modulates immune activity, promotes nerve growth factor expression, accelerates stem cell differentiation into neurons, and aids tissue regeneration. This study presents a novel wireless charging magnetoelectric (ME) scaffold, contributing to address critical gap in therapeutic strategies for PNI.