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Bi-directionalized Promoter Systems Allow Methanol-Free Production of Hard-to-express Peroxygenases with Komagataella Phaffii

Mihail Besleaga,Christian Zimmermann,Katharina Ebner,Robert L. Mach,Astrid R. Mach-Aigner,Martina Geier,Anton Glieder,Oliver Spadiut,Julian Kopp

Microbial Cell Factories（2024）

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Key words
Komagataella phaffii,Bi-directionalized promoter,Unspecific peroxygenase,Recombinant protein production,Methanol-free,Derepressed feeding,UPR,ERAD
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