Movie S5 from Macrophage-Mediated Trogocytosis Leads to Death of Antibody-Opsonized Tumor Cells

Live cell imaging of macrophage-mediated trogocytosis. The movie shows trastuzumab from live, opsonized MDA-MB-453 cells being trogocytosed by CD45-labeled J774A.1 macrophages. Upper left panel shows signal from Alexa 555-labeled trastuzumab, upper right panel shows CD45 signal, lower left panel shows the transmitted light image and the lower right image shows the overlay of the upper two panels. The sample was imaged in the presence of caspase 3/7 detection reagent for which the signal is also detected in the CD45 channel. The movie plays at a speed of 340x real-time. Scale bar = 5 µm.