Supplement S-1. Supplementary Table 1. Supplementary Figures 1-9. from Genetic Determinants of 1,3-Butadiene Metabolism and Detoxification in Three Populations of Smokers with Different Risks of Lung Cancer

Experimental details Supplement S-1. Details of HPLC-ESI-MS/MS methodology. Tables Supplementary Table 1. Geometric means (95% confidence limits) for urinary MHBMA and DHBMA by race/ethnicity and sex. Values are given in ng/mg creatinine (Cr). Figures Supplementary Figure 1. Extracted ion chromatograms showing formation of EB-GSH (m/z 378.2) in the presence of GSTT1 (A) and GSTT2 (B). Regional Association Plots Supplementary Figure 2. LocusZoom plot of the GSTT1 region on chromosome 22 with MHBMA results in our overall multi-ethnic sample. The color scheme indicates linkage disequilibrium (LD) structure across the region, using African LD values. The blue line indicates recombination rate. P-values are -log transformed. Rs6004031, the top-most association, is marked by a purple diamond (P-value 1.26x10-23). Supplementary Figure 3. LocusZoom plot of Chromosome 22 results, adjusted for GSTT1 deletion, with MHBMA results in our multi-ethnic sample. The color scheme indicates linkage disequilibrium (LD) structure across the region, using European LD values. The blue line indicates recombination rate. P-values are -log transformed. Rs2070767, the top-most association is marked by a purple diamond (P-value 2.7x10-4). Supplementary Figure 4. LocusZoom plot of the GSTT1 region with MHBMA results from the African American ethnic specific analysis. The color scheme indicates linkage disequilibrium (LD) structure across the region, using African LD values. The blue line indicates recombination rate. P-values are -log transformed. Rs5760092, the top-most association, is marked by a purple diamond (P-value 2.6x10-9). Supplementary Figure 5. LocusZoom plot of the GSTT1 region with MHBMA results from the Japanese American ethnic specific analysis. The color scheme indicates linkage disequilibrium (LD) structure across the region, using Asian LD values. The blue line indicates recombination rate. P-values are -log transformed. Rs6004031, the top-most association, is marked by a purple diamond (P-value 5.13x10-9). Supplementary Figure 6. LocusZoom plot of the GSTT1 region with MHBMA results from the White ethnic specific analysis. The color scheme indicates linkage disequilibrium (LD) structure across the region, using European LD values. The blue line indicates recombination rate. P-values are -log transformed. Rs72617242, the top-most association, is marked by a purple diamond (P-value 9.5x10-15). Quantile-Quantile Plots Supplementary Figure 7. Quantile-Quantile plot of observed and expected -log10 transformed p-values of associations with MHBMA values near the GSTT1 gene. Supplementary Figure 8. Quantile-Quantile plot of observed and expected -log10 transformed p-values of associations with DHBMA values near the GSTT1 gene Supplementary Figure 9. Quantile-Quantile plot of observed and expected -log10 transformed p-values of associations with the metabolic ratio values near the GSTT1 gene. The metabolic ratio is calculated as DHBMA [MHBMA / (MHBMA + DHBMA)]