FIGURE 5 from Neoadjuvant CD40 Agonism Remodels the Tumor Immune Microenvironment in Locally Advanced Esophageal/Gastroesophageal Junction Cancer

Sotigalimab results in immunomodulation and apoptosis of tumor cells. A, Tumor cells in the TME were characterized using MIBI analysis of keratin, HLA-I, HLA-II, PD-L1, and CD40 (representative image: patient 04). B, Quantification of tumor cell density by MIBI (pre n = 5, post n = 4). C–E, Expression of the phenotypic markers was also quantified in the MIBI analysis for HLA I and II (C), PD-L1 (D), and CD40 (E; pre n = 5, post n = 4). F–H, Differential expression by timepoint using scRNAseq of CD274 (F), HLA I and II genes (G), and apoptosis genes (H) on tumor cells. For scRNAseq data, comparisons denoted as significant have adjusted P < 0.05, and those without an asterisk are not significant.