Whole genome sequencing and analysis of Inonotus hispidus isolated from the Chengde Mountain Resort, China

Abstract Inonotus hispidus is an edible medicinal macrofungus whose nutritional and therapeutic qualities are well-known. The species is widely distributed in Asia, Europe, North Africa, and North America. As a medicinal and health resource it is highly valued in East Asian countries, notably China, Japan, and South Korea. The antiviral, antioxidant, antitumor, hypolipidemic, and immunomodulatory activity of I. hispidus have been documented. In this study, we utilized a combination of Illumina NovaSeq and PacBio Sequel platforms to sequence the whole genome of a strain of I. hispidus named "CDMR" after the Chengde Mountain Resort (Hebei Province, China), where the spores of fresh wild fruiting bodies were collected. The genome size of I. hispidus CDMR was found to be 34.22 Mb, containing 7,723 coding genes, 8,100 repetitive sequences, 91 tRNAs, 13 rRNAs, and 15 snRNAs. Sets of these genes were annotated using the following databases: GO (5,012 genes), KEGG (5,714), COG (1,209), NR (6,576), TCDB (297), InterPro (5,012), and Uniprot (1,639). In addition, we found 313, 340, 128, 116, and 728 genes related to carbohydrate-active enzymes, secretory proteins, secondary metabolism, Cytochrome P450, and pathogen–host interactions, respectively. Our findings offer comprehensive insights into the genome of I. hispidus CDMR, fostering the exploration of genomic and functional variances among I. hispidus populations from diverse regions. Moreover, this study contributes to the advancement of the genetic and genomic investigations of the nutritional and therapeutic attributes of I. hispidus.