Green Synthesis of CuO Nanoparticles Using Ligustrum Lucidum Extract, and the Antioxidant and Antifungal Evaluation

Biosynthesis of metal oxide nanoparticles using plant extract is an inexpensive, simple, rapid, and environmentally friendly approach to obtaining nanoparticles for biological applications. Herein, copper oxide nanoparticles (CuO-NPs) were successfully synthesized using an aqueous extract from Ligustrum lucidum leaves. The structural, optical, and morphological characteristics of the nanoparticles were assessed using x-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, UV-visible spectrophotometer, transmission and scanning electron microscopy (TEM and SEM), and energy-dispersive x-ray (EDX). Nanocrystalline CuO with an average crystalline size of 22.0 nm and a band gap energy of 1.4 eV were confirmed from the XRD and UV-vis spectrophotometer, respectively. Morphological studies showed spherical nanoparticles, whose particle size estimation (30 +/- 5 nm) agrees with the crystalline size deduced from the XRD pattern. A free radical scavenging activity of the CuO nanoparticles, evaluated using the 1, 1-diphenhyl-2-picrylhydrazyl (DPPH) assay, showed that it exhibited high antioxidant activity (IC50: 63.35 mu g ml-1) that is concentration dependent. Antifungal evaluation using four different fungal strains (Aspergillus flavus, Aspergillus niger, Fusarium oxysporum, and Trichoderma harzianum) indicated a direct relationship between the potency of the particles and their concentration, with 1 ppm solution exhibiting the highest potency. The green synthesized CuO-NPs using Ligustrum lucidum may be potentially used as an antioxidant and antifungal agent for therapeutic applications.