谷歌浏览器插件
订阅小程序
在清言上使用

Immune-profiling of T helper 1 (Th1), Th2 and Th17 signatures in murine splenocytes by targeting intracellular cytokines

Soumik Barman, Aisling Kelly,Danica Dong, Arsh Patel, Michael J. Buonopane, Jake Gonzales, Ben Janoschek, Andrew Draghi,David J. Dowling

crossref(2024)

引用 0|浏览7
暂无评分
摘要
Functional cytokines shape both innate and adaptive immune responses in the host after infection or immunization. Deep immunophenotyping of the key functional cytokine signatures associated with T cells in murine lymphoid tissue, especially in the spleen, is challenging. Using spectral flow cytometry, we developed a 17-parameter panel to profile major immune cell subsets along with T cells, memory phenotypes and functional cytokines in murine splenocytes in steady state as well as in stimulated conditions. This panel dissects the memory T cell compartment via CD62L and CD44 expression after mitogen stimulation. To profile T helper (Th) cells distribution after mitogen stimulation, established Th1 markers IFNγ, TNF and IL-2; Th2 markers IL-4/5 and the Th17 marker, IL-17, are included. This optimized multicolor spectral flow panel allows a detailed immune profiling of functional cytokines in the murine T cell compartment and might be useful for exploratory analysis of how these functional cytokines shape host immunity after infection or vaccination. Our panel could be easily modified, if researchers wish to tailor the panel to their specific needs. ### Competing Interest Statement Ben Janoschek and Andrew Draghi II are employees of Sony Biotechnology Inc., the manufacturer of the Sony ID7000 flow cytometer used in this study. Jake Gonzales is an employee of BioLegend Inc. David J. Dowling is a named inventors on patents relating to small molecule adjuvants assigned to Boston Childrens Hospital, is on the scientific advisory board of EdJen BioTech and serves as a consultant with Merck Research Laboratories/Merck Sharp & Dohme Corp. (a subsidiary of Merck & Co., Inc.). David J. Dowling is cofounder of Ovax Inc. The other authors declare no conflict of interest.
更多
查看译文
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要