Biochemical assay for detection of pathogenic and probiotic bacteria at shrimp and prawn from wild and different culture conditions in bangladesh

We conducted biochemical assays on shrimp and prawn samples in Bangladesh, revealing the presence of both harmful and beneficial bacteria. Pathogens such as Escherichia coli, Klebsiella typhimurium, Shigella flexneri, Listeria monocytogenes, and Staphylococcus aureus were detected in these samples, with Escherichia coli being the most prevalent. Additionally, we identified three potential probiotic bacteria - Bacillus subtilis, Lactobacillus spp., and Enterobacter aerogenes. These probiotics can enhance immunity, support survival, and have minimal environmental impact. Shrimp and prawn farms in Bangladesh may be susceptible to harmful microorganisms due to contaminated water sources and subpar sanitation during post-harvest handling and processing. Our use of biochemical characterization proves a dependable method for distinguishing pathogenic and probiotic bacteria in shrimp and prawns, offering valuable insights for future disease research.