PD25-08 MEDIHONEY AND MĀNUKA HONEY EXTRACT INHIBIT THE ACTIVATION OF THE MAST CELL RELATED-G-PROTEIN COUPLED RECEPTOR X2 (MRGPRX2): POTENTIAL INTRAVESICAL USE IN PATIENTS WITH INFLAMMATORY BLADDER PATHOLOGY

You have accessJournal of UrologyInfections/Inflammation/Cystic Disease of the Genitourinary Tract: Interstitial Cystitis (PD25)1 May 2024PD25-08 MEDIHONEY AND MĀNUKA HONEY EXTRACT INHIBIT THE ACTIVATION OF THE MAST CELL RELATED-G-PROTEIN COUPLED RECEPTOR X2 (MRGPRX2): POTENTIAL INTRAVESICAL USE IN PATIENTS WITH INFLAMMATORY BLADDER PATHOLOGY Omar Abdelwahab, Brian Birch, Oliver Miller, Prapussara Sirikhansaeng, Laurie Lau, Andrew Walls, Troy Merry, Jackie Evans, and Bashir Lwaleed Omar AbdelwahabOmar Abdelwahab , Brian BirchBrian Birch , Oliver MillerOliver Miller , Prapussara SirikhansaengPrapussara Sirikhansaeng , Laurie LauLaurie Lau , Andrew WallsAndrew Walls , Troy MerryTroy Merry , Jackie EvansJackie Evans , and Bashir LwaleedBashir Lwaleed View All Author Informationhttps://doi.org/10.1097/01.JU.0001008584.88541.ff.08AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Mast cells numbers and activity are significantly elevated in several inflammatory conditions including interstitial cystitis (IC/BPS)This condition is associated with the release of neuroactive substances, e.g. substance P (SP), which induce mast cell degranulation and tissue inflammationMedihoney® (MH) is a medical grade Mānuka honey with strong anti-microbial properties. Recent reports demonstrate its anti-inflammatory properties via inhibition of mast cell histamine releaseIn the present study, we investigated the anti-inflammatory effect of MH and a sugar free Mānuka honey extract (EX) against neurogenic inflammation by studying their effects on SP-induced mast cell degranulation via its associated receptor MRGPRX2. METHODS: LAD2 mast cells were activated with SP with or without pre-incubation with MH or EX at 4, 2 and 1% concentrations. 1. Degranulation was assessed via a β-hexosaminidase release assay. 2. The effect of MH and EX on MRGPRX2 activation was assessed via Fluo-4 Direct™ Calcium Assay in HEK-293 cells transfected to express MRGPRX2 using the Flexstation II 384 platform. RESULTS: 1. Pre-incubation of LAD2 cells with MH or EX induced strong inhibition of SP-induced β-hexosaminidase release (Figure 1A & B)2. In addition, both MH and EX induced 77% and 62% drops in substance P-induced Calcium flux, respectively in the transfected HEK-293 cells (Figure 1 C)These effects are independent of the sugars naturally occurring in MH and both effects were maximal at 4% concentration. CONCLUSIONS: Both MH and EX exert a strong dose-dependent inhibition of 1. degranulation in LAD2 cells and2. MRGPRX2 activation in transfected HEK-293 cells. Based on our results, MH and EX (used intravesically) could potentially be useful as anti-inflammatory agents directed against the neurogenic inflammation involved in the pathophysiology of IC/BPS. Download PPT Source of Funding: PhD degree funding © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e539 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Omar Abdelwahab More articles by this author Brian Birch More articles by this author Oliver Miller More articles by this author Prapussara Sirikhansaeng More articles by this author Laurie Lau More articles by this author Andrew Walls More articles by this author Troy Merry More articles by this author Jackie Evans More articles by this author Bashir Lwaleed More articles by this author Expand All Advertisement PDF downloadLoading ...