MP67-08 BIOCHEMICAL AND HISTOPATHOLOGICAL COMPARISON OF THE EFFECTS OF RENAL ARTERY VEIN CLAMPING AND RENAL ARTERY ONLY CLAMPING DURING PARTIAL NEPHRECTOMY IN RATS WITH SOLITARY KIDNEY

You have accessJournal of UrologyKidney Cancer: Localized: Surgical Therapy V (MP67)1 May 2024MP67-08 BIOCHEMICAL AND HISTOPATHOLOGICAL COMPARISON OF THE EFFECTS OF RENAL ARTERY VEIN CLAMPING AND RENAL ARTERY ONLY CLAMPING DURING PARTIAL NEPHRECTOMY IN RATS WITH SOLITARY KIDNEY Tunahan Ates, Mutlu Deger, Nebil Akdogan, Ismail Onder Yilmaz, Ozlem Goruroglu Ozturk, Tugba Toyran, Seyda Erdogan, Volkan Izol, and Ibrahim Atilla Aridogan Tunahan AtesTunahan Ates , Mutlu DegerMutlu Deger , Nebil AkdoganNebil Akdogan , Ismail Onder YilmazIsmail Onder Yilmaz , Ozlem Goruroglu OzturkOzlem Goruroglu Ozturk , Tugba ToyranTugba Toyran , Seyda ErdoganSeyda Erdogan , Volkan IzolVolkan Izol , and Ibrahim Atilla AridoganIbrahim Atilla Aridogan View All Author Informationhttps://doi.org/10.1097/01.JU.0001009496.54470.10.08AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: The standard surgical treatment of renal masses is radical nephrectomy (RN) or partial nephrectomy (PN). PN is performed with (vascular clamping) or without ischemia. In the literature, the superiority of artery only (AO) clamping over artery-vein (AV) clamping has been investigated. We aimed to investigate whether AO clamping or AV clamping is superior by performing an animal experiment. METHODS: A total of 24 animals were divided into 3 groups: Control (C) group, artery only (AO) group and artery-vein (AV) group. All rats underwent right nephrectomy first. Then, left nephrectomy was performed 6 days later in the C group, left PN was performed 3 days later in the AO group by clamping the AO, and left PN was performed 3 days later in the AV group by clamping the AV. The experiment was then terminated. Total antioxidant status, total oxidant status, oxidative stress index, malondialdehyde acid, super oxide dismutase, nitric oxide, tyrosine hydroxylase, 8-isoprostane, creatinine, interleukin-6 were determined from rat sera with ELISA kits. In left nephrectomy materials, Bowman's capsule enlargement, tubular necrosis, vasa recta leukocyte infiltration (VRLI), tubular regeneration, interstitial inflammation, tubular dilatation and presence of mitosis were examined histopathologically. Markers and histopathologic findings were compared.Chi-square test or Fisher's test was used to compare categorical variables, Kruskal Wallis test was used to compare continuous measurements and Mann Whitney U Test was used for post-hoc comparisons. The p level was taken as 0.017 for significance with Bonferroni correction. RESULTS: There was no statistically significant difference between the groups in terms of biochemical measurements. In the comparison of Bowman's capsule enlargement, tubular necrosis, mitosis, VRLI, and tubular dilatation; statistically significant difference was found between ischemia groups and control groups. CONCLUSIONS: This study demonstrated that AO and AV clamping yielded similar results. According to the current situation, the decision of the appropriateness of AO or AV clamping should be left to the surgeon. Source of Funding: Çukurova University Scientific Research Projects © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e1099 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Tunahan Ates More articles by this author Mutlu Deger More articles by this author Nebil Akdogan More articles by this author Ismail Onder Yilmaz More articles by this author Ozlem Goruroglu Ozturk More articles by this author Tugba Toyran More articles by this author Seyda Erdogan More articles by this author Volkan Izol More articles by this author Ibrahim Atilla Aridogan More articles by this author Expand All Advertisement PDF downloadLoading ...