PD12-11 DISRUPTION OF METABOLIC AND URINARY FUNCTIONS IN MICE INDUCED BY CHRONIC MEALTIME SHIFT: IMPACT OF DAILY ANTIOXIDANT SUPPLEMENTATION

You have accessJournal of UrologyBladder & Urethra: Anatomy, Physiology & Pharmacology (PD12)1 May 2024PD12-11 DISRUPTION OF METABOLIC AND URINARY FUNCTIONS IN MICE INDUCED BY CHRONIC MEALTIME SHIFT: IMPACT OF DAILY ANTIOXIDANT SUPPLEMENTATION Chung Lyul Lee, Hyun Seok Na, Ki Hak Song, Jae Sung Lim, Ju Hyun Shin, Jong Mok Park, Ji Yong Lee, Jae Geun Lee, Yong Gil Na, and Khae Hwan Kim Chung Lyul LeeChung Lyul Lee , Hyun Seok NaHyun Seok Na , Ki Hak SongKi Hak Song , Jae Sung LimJae Sung Lim , Ju Hyun ShinJu Hyun Shin , Jong Mok ParkJong Mok Park , Ji Yong LeeJi Yong Lee , Jae Geun LeeJae Geun Lee , Yong Gil NaYong Gil Na , and Khae Hwan KimKhae Hwan Kim View All Author Informationhttps://doi.org/10.1097/01.JU.0001008772.30001.48.11AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: In the context of the prevalent circadian rhythm disorders among modern society, particularly among shift workers, this study aimed to investigate the impact of urinary rhythm disturbances caused by dietary changes typical of night shift workers in mice. METHODS: Three distinct groups were established based on feeding schedules: ad libitum feeding (AF), daytime feeding (DF), and restrictive feeding (RF) groups. Urinary rhythm was examined in each group, along with mRNA rhythm alterations in bladder tissue components. Additionally, urination rhythm was analyzed in per1 and per2 double knock-out (PDK) mice, and the influence of mealtime shift on urination rhythm in WT mice was investigated following antioxidant injection. RESULTS: The study revealed significant disruptions in circadian patterns of water intake and urinary excretion due to mealtime shifts. Experiments with Per2::Luc knock-in mouse bladders cultured ex vivo demonstrated increased amplitude and delayed acrophases of Per2 oscillation in response to mealtime shifts. Daily antioxidant supplementation did not mitigate the mealtime shift-induced circadian patterns of water intake, urinary excretion, or altered Per2 oscillation patterns in cultured mouse bladders. However, in the case of aged mice, antioxidants partially restored urinary rhythm. CONCLUSIONS: The study elucidates the significant impact of mealtime shifts on mouse urination rhythm, regardless of the presence of circadian clock genes. While antioxidants did not fully counteract these disruptions, they exhibited partial restoration of urinary rhythm in aged mice. These findings contribute valuable insights into the understanding of circadian disruptions induced by mealtime shifts, shedding light on potential interventions for mitigating associated health risks. Download PPTDownload PPT Source of Funding: None © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e260 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Chung Lyul Lee More articles by this author Hyun Seok Na More articles by this author Ki Hak Song More articles by this author Jae Sung Lim More articles by this author Ju Hyun Shin More articles by this author Jong Mok Park More articles by this author Ji Yong Lee More articles by this author Jae Geun Lee More articles by this author Yong Gil Na More articles by this author Khae Hwan Kim More articles by this author Expand All Advertisement PDF downloadLoading ...