A Combination of Β-Aescin and Newly Synthesized Alkylamidobetaines As Modern Components Eradicating the Biofilms of Multidrug-Resistant Clinical Strains of Candida Glabrata

Emil Paluch,Olga Bortkiewicz,Jaroslaw Widelski,Anna Duda-Madej,Michal Glensk,Urszula Nawrot,Lukasz Lamch, Daria Dlugowska,Beata Sobieszczanska,Kazimiera A. Wilk

International journal of molecular sciences（2024）SCI 2区SCI 3区

Wroclaw Med Univ | Lublin Med Univ | Wroclaw Univ Sci & Technol

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Abstract

The current trend in microbiological research aimed at limiting the development of biofilms of multidrug-resistant microorganisms is increasingly towards the search for possible synergistic effects between various compounds. This work presents a combination of a naturally occurring compound, β-aescin, newly synthesized alkylamidobetaines (AABs) with a general structure—CnTMDAB, and antifungal drugs. The research we conducted consists of several stages. The first stage concerns determining biological activity (antifungal) against selected multidrug-resistant strains of Candida glabrata (C. glabrata) with the highest ability to form biofilms. The second stage of this study determined the activity of β-aescin combinations with antifungal compounds and alkylamidobetaines. In the next stage of this study, the ability to eradicate a biofilm on the polystyrene surface of the combination of β-aescin with alkylamidobetaines was examined. It has been shown that the combination of β-aescin and alkylamidobetaine can firmly remove biofilms and reduce their viability. The last stage of this research was to determine the safety regarding the cytotoxicity of both β-aescin and alkylamidobetaines. Previous studies on the fibroblast cell line have shown that C9 alkylamidobetaine can be safely used as a component of anti-biofilm compounds. This research increases the level of knowledge about the practical possibilities of using anti-biofilm compounds in combined therapies against C. glabrata.

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Key words

beta-aescin,newly synthesized alkylamidobetaines,surface-active compounds,surfactant,multidrug resistant,Candida glabrata,biofilm,biological activity

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