Role of Lipocalin-2 in N1/N2 Neutrophil Polarization After Stroke

Background Neutrophils and Lipocalin-2 (LCN2) play pivotal roles in cerebral ischemia-reperfusion (I/R) injury. However, their contribution is not fully clarified.Objective This study aimed to explore the role of LCN2 and its association with neutrophil polarization in I/R injury.Methods A mouse model of middle cerebral artery occlusion (MCAO) was used to induce cerebral ischemia. LCN2mAb was administered 1 h and Anti-Ly6G was administered for 3d before MCAO. The role of LCN2 in the polarity transition of neutrophils was explored using an in vitro HL-60 cell model.Results LCN2mAb pretreatment had neuroprotective effects in mice. The expression of Ly6G was not significantly different, but the expression of N2 neutrophils was increased. In the in vitro study, LCN2mAb-treated N1-HL-60 cells induced N2-HL-60 polarization.Conclusion LCN2 may affect the prognosis of ischemic stroke by mediating neutrophil polarization.