AIEE active Cholyl Hydrazide Carbazole-Schiff base for selective detection of copper (II) ion

A novel aggregation-induced emission enhancement (AIEE) active Schiff-based fluorescent probe Cholyl Hydrazide Carbazole-Schiff base (ChHCz-SB) was synthesized using cholyl hydrazide and carbaldehyde, and its properties were assessed using a range of spectroscopic methods. In a 70 % water-THF mixture, the ChHCz-SB showed the highest fluorescence intensity, which was 5.93 times greater than in pure THF. It was discovered that in the presence of different cations and anions. ChHCz-SB was a sensitive and selective "turn-off" fluorescence detector for Cu2} metal ions. The binding constant (Ka) and limit of detection (LOD) were found to be 5.3 x 107 M-1 and 1.59 x 10-7 M, respectively. Job's plot was used to determine the 1:1 binding stoichiometry between copper (II) metal ion and ChHCz-SB. Moreover, Cu2} metal ion concentration in various water samples was successfully measured using the fluorescent probe ChHCz-SB. Furthermore, the distribution of electronic states in the Schiff base and the sensing mechanism were investigated through Density functional theory (DFT) calculations of ChHCz-SB.