Molecular Mechanism of Efficient Degradation of TPM Dyes by Serratia Species WKD at High Temperature

Understanding the molecular mechanism of bacterial triphenylmethane (TPM) dyes degradation is crucial for its practical application. Herein, genome and transcriptome analysis was used to investigate the molecular mechanism of efficient TPM dyes degradation by Serratia sp. strain WKD, including at high temperatures. Genome analysis results indicated that the cytochrome p450 gene, aromatic compound degradation gene, and some other metabolic degradation genes were probably involved in TPM dyes degradation. Heat shock protein genes, and motility and chemotaxis genes might be critical for the strain to adapt to high temperatures. Furthermore, RNA-Seq and qRT-PCR analysis results confirmed that frmA and copA were the key genes involved in malachite green (MG) and crystal violet (CV) degradation at 30 degrees C. Besides, heat shock protein genes ibpB and ibpA, and lon, amiD_2, and cueO (frmA) were the key genes involved in MG (CV) degradation at high temperatures (45 degrees C). Moreover, molecular mechanism of efficient MG and CV degradation by the strain was proposed, including at high temperatures. These results provide a valuable theoretical basis for practical application.