Ionic Liquid-Enhanced Silica Aerogels for the Specific Extraction and Detection of Aflatoxin B1 Coupled with a Smartphone-Based Colorimetric Biosensor

The polymer ionic liquid (1-allyl-3-butylimidazolium bromide) enhanced silica aerogel was modified onto the surface of stainless-steel mesh to immobilize aptamer-1 for the specific recognition of AFB1. The porous channels of silica aerogel could prevent the interference of macromolecules in food samples. Enzyme kinetic analysis showed that the MoS 2 /Au was an effective peroxidase mimic with a relatively low Michaelis constant ( K m ) value of 0.17 mM and a high catalytic rate of 3.87 x 10 -8 mol (L & sdot;s) - 1 , which exhibited obvious superiority compared with horseradish peroxidase. The established "sandwich-structure" biosensor was coupled with the smartphone "Color Picker" application was used to detect AFB1 with a wide linear range (1-100 ng mL -1 ) and low detection limit (0.25 ng mL -1 ). The anti-interference ability of the established biosensor was evaluated by adding different concentrations of standards in corn, peanut, and wheat and matrix effects were 90.84-106.11 %. The results showed that this method demonstrated high specificity, sensitivity, rapidity and low interference in food samples.