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Molecular Cloning, Biophysical and in Silico Studies of Human Papillomavirus 33 E2 DNA Binding Domain.

Bharti,Maya S. Nair

Journal of biomolecular structure and dynamics/Journal of biomolecular structure & dynamics(2024)

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摘要
Human papillomavirus 33, a high-risk HPV strain, is mainly responsible for HPV infection and cervical cancer in Asian countries. The E2 protein of HPV 33 is a DNA-binding protein that plays a crucial role in viral replication and transcription. We have cloned, overexpressed, and purified the DNA binding domain of the E2 protein. Size exclusion chromatography results suggested that the protein exists in a homodimeric state in the native form. Circular dichroism data showed that the protein has a higher content of beta-sheet. The melting temperature obtained from differential scanning calorimetry is 52.59 degrees C, and the protein is stable at pH 8 and is in a dimeric form at basic pH. The protein is monomeric or unfolded at a very low pH. Chemical denaturation studies suggested that the protein denatured and dissociated simultaneously. The DNA binding activity of the protein was also confirmed and it showed binding affinity in the order of 10(6) M-1. The protein structure was modeled using homology modeling and other bioinformatic tools. The virtual screening and molecular dynamic simulation studies were performed to find compounds that can act as potent inhibitors against E2 DBD. This study expands the understanding of the conserved structural and binding properties of HPV33 E2 DBD and provides the first report on the characterization of the viral protein. [GRAPHICS]
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关键词
Human papillomavirus,size exclusion chromatography,DNA binding domain,fluorescence,circular dichroism,homology modeling,molecular dynamic simulations
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