Assembly of Printed Interconnects for Immobilized Protein Microfluidic Assays

Microfluidics has attracted significant attention for biological applications, particularly for high-throughput screening and multiplexing. However, conventional interconnect techniques are limited in their ability to fabricate the hundreds or thousands of microfluidic interconnects needed to process multiple samples for high-throughput microfluidics. To address this challenge, coaxial printing was invented to enable rapid, customizable, and scalable microfluidic interconnects. This paper focuses on the assembly process of coaxial printed interconnects for immobilized protein microfluidic assays. We demonstrate the immobilization of protein into a 1 × 1 microwell microfluidic unit with printed connectors to showcase the process.