Response of Different Genotypes on in Vitro Regeneration of Maize (zea Mays L.)

In the present experiment the regeneration of different genotypes of maize towards a specific media was studied. The response of the explants on the culture media is genotype dependent due to which the in vitro regeneration of the plant varies with respect to its genotype. Maize genotypes have profound differences for in vitro cultures (Armstrong and Green, 1985) and only small number of maize genotype posses’ regeneration capacity. With the help of this, the genetic variability of the plant can be studied and the elite genotypes of maize can be manipulated with the desirable traits using the genetic engineering technology. In the present investigation three different genotypes namely, two hybrids K-25 and 4212; and a composite genotype BVM-2 were used. Mature embryo was used as the explants. Kernels of the three genotypes were surface sterilised and soaked in double distilled water and kept at 10-14°C for 4 days. Explants of all the genotypes were cultured in the N6 media. The data recorded on the three genotypes were subjected to the statistical analysis following Completely Randomised Design (CRD). Significant results were obtained which showed that the hybrid genotype K-25 gave the highest frequency of callusing with 66.66%.The result obtained suggest that the hybrid K-25 can be used for genetic engineering in furthering sustainable food requirement for the growing population and economic growth.