Bovine Endothelial Cells Interact with Fully-luteinized, but Not Luteinizing, Granulosa Cells in the Mrna Expression of Endothelin-1 System in Response to Prostaglandin F2α

Reproduction in Domestic AnimalsVolume 42, Issue 6 p. 637-642 Bovine Endothelial Cells Interact with Fully-luteinized, but Not Luteinizing, Granulosa Cells in the mRNA Expression of Endothelin-1 System in Response to Prostaglandin F2α K Shirasuna, K Shirasuna Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, JapanSearch for more papers by this authorS Watanabe, S Watanabe Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, JapanSearch for more papers by this authorD Yamamoto, D Yamamoto Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, JapanSearch for more papers by this authorM Hayashi, M Hayashi Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, JapanSearch for more papers by this authorK Nagai, K Nagai Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, JapanSearch for more papers by this authorA Miyamoto, A Miyamoto Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, JapanSearch for more papers by this author K Shirasuna, K Shirasuna Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, JapanSearch for more papers by this authorS Watanabe, S Watanabe Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, JapanSearch for more papers by this authorD Yamamoto, D Yamamoto Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, JapanSearch for more papers by this authorM Hayashi, M Hayashi Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, JapanSearch for more papers by this authorK Nagai, K Nagai Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, JapanSearch for more papers by this authorA Miyamoto, A Miyamoto Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, JapanSearch for more papers by this author First published: 29 October 2007 https://doi.org/10.1111/j.1439-0531.2006.00835.xCitations: 3 Author's address (for correspondence): A Miyamoto, Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan. E-mail: akiomiya@obihiro.ac.jp Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Contents The corpus luteum (CL) undergoes regression by prostaglandin (PG)F2α from uterus and endothelin-1 (ET-1) plays an important role during luteolysis as a local mediator of PGF2α in the cow. Endothelial cells (EC) and luteal cells are main cell types making up the CL and their interactions are vital for CL function. We aimed to examine the relevance of interactions between EC and luteal cells on stimulation of genes which involved ET-1 synthesis by PGF2α. We further focused the impact of maturity of luteal cells on the stimulation of the genes. To make a microenvironment which resembles the CL, we used bovine aortic endothelial cells (BAEC) and luteinizing or fully-luteinized granulosa cells (GC) and evaluated the effect of PGF2α on the expression for mRNA of ET-1 system by using real-time RT-PCR. PGF2α stimulated the expression of preproET-1 and endothelin converting enzyme-1 mRNA only in the co-cultures of BAEC with fully-luteinized GC, but not with luteinizing GC. The data suggest that interactions between BAEC and fully-luteinized GC enhance the capability of BAEC to produce ET-1 in response to PGF2α. This mechanism may contribute to the local induction of luteolytic action of PGF2α which is dependent on the age/maturation of the CL. Citing Literature Volume42, Issue6December 2007Pages 637-642 RelatedInformation