Phosphonium-Substituted Conjugated Polyelectrolytes Display Efficient Visible-Light-Induced Antibacterial Activity.

Advanced Computer Systems (ACS)(2024)

Univ Texas San Antonio

Cited 1|Views7
Abstract
We report the light-activated antibacterial activity of a new class of phosphonium (R-PMe3+)-substituted conjugated polyelectrolytes (CPEs). These polyelectrolytes feature a poly(phenylene ethynylene) (PPE) conjugated backbone substituted with side groups with the structure -O-(CH2)(n)PMe3+, where n = 3 or 6. The length of the side groups has an effect on the hydrophobic character of the CPEs and their propensity to interact with bacterial membranes. In a separate study, these phosphonium-substituted PPE CPEs were demonstrated to photosensitize singlet oxygen (O-1(2)) and reactive oxygen species, a key factor for the photoinduced inactivation of bacteria. In this study, in vitro antibacterial assays against Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus were performed by employing the series of polyelectrolytes under both dark and illumination conditions. In general, the phosphonium-substituted CPEs displayed profound light-activated biocidal activity, with >99% colony forming unit (CFU) reduction after 15 min of light exposure (16 mW cm(-2)) at a <= 20 mu M CPE concentration. Strong biocidal activity was also observed in the dark for a CPE concentration of 20 mu M against S. aureus; however, higher concentrations (200 mu M) were needed to enable dark inactivation of E. coli. The dark activity is ascribed to bacterial membrane disruption by the CPEs, supported by a correlation of dark biocidal activity with the chain length of the side groups. The light-activated biocidal activity is associated with the ability of the CPEs to sensitize ROS, which is cytotoxic to the microorganisms. Serial dilution bacterial plating experiments revealed that the series of CPEs was able to induce a >5-log kill versus E. coli with 15 min of exposure to a blue LED source (16 mW cm(-2)).
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Key words
conjugated polyelectrolytes,antimicrobial polymers,singlet oxygen,phosphoniumfunctionality,photodynamic therapy,photosensitizer,Staphylococcus aureus,Escherichia coli
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要点】:本研究报道了一种新型磷onium取代的共轭聚电解质(CPEs)在可见光照射下具有高效的抗菌活性,其侧链长度影响其在黑暗和光照条件下的抗菌效果。

方法】:通过合成具有不同侧链长度的磷onium取代的聚(苯基乙炔)共轭骨架CPEs,并研究其与细菌细胞膜的相互作用及光诱导活性氧种产生能力。

实验】:在暗条件和光照条件下,采用E. coli和S. aureus进行体外抗菌实验,使用系列CPEs,数据集名称未提及。实验结果表明,在15分钟蓝光LED照射(16 mW cm(-2))下,≤20 μM浓度的CPEs可减少>99%的CFU。在黑暗条件下,20 μM的CPEs对S. aureus表现出强抗菌活性,而对E. coli则需要200 μM的浓度。通过细菌平板稀释实验发现,CPEs在15分钟蓝光照射下可对E. coli实现>5-log的杀灭效果。