Supplementary Figures 1 - 7 from Clusterin is a Gene-Specific Target of Microrna-21 in Head and Neck Squamous Cell Carcinoma

PDF file - 133K, Supplementary Figure 1. miRNA-21 is upregulated in HNSCC tumors. Supplementary Figure 2. RT-qPCR validation of miRNA-21 overexpression by transient transfection. (A) pre-miRNA-21 and (B) miRNA-21 expression level. Supplementary Figure 3. miRNA-21 overexpression stimulates cell proliferation. All measurements were obtained with the Cell Counting Kit -8 cell proliferation assay. ∗p<0.01. Supplementary Figure 4. miRNA-21 loses specificity for CLU transcript when miRNA-21 binding seed sequence is deleted from the CLU 3'UTR sequence. ∗p<0.03. Supplementary Figure 5. Relative expression of CLU-1 in normal and HNSCC cell lines. Supplementary Figure 6. RT-qPCR validation of CLU-1 overexpression by transient transfection. Supplementary Figure 7. CLU-1 has growth inhibitory effects in JHU-O28, FaDu and SCC9 HNSCC cell lines. Overexpression of CLU-1 inhibits cell proliferation. All measurements were obtained by taking direct cell counts at each time point. ∗p<0.05.