CIR-17-0171 Supplementary Data from CD28 and 41BB Costimulation Enhances the Effector Function of CD19-Specific Engager T Cells

S1: T-cell immunophenotyping by flow cytometry. S2: Determination of TSCM presence in PBMC and NT T-cells by flow cytometry. S3: CD19-ENGT-cells and CD19-ENGT-cells expressing CD80 and/or 41BBL kill Nalm 6 cells efficiently. S4: Experimental set up of Serial Killing Assay. S5: CD19- ENG.41BBL/CD80 T-cells have improved ability to sequentially kill CD19 target cells. S6: CD19-ENG T-cells expressing CD80 or 41BBL and CD80 have improved ability to sequentially kill CD19 target cells. S7: Nalm 6 express PD-L1 after exposure to IFNgamma. S8: CD19 ENG.41BBL/CD80 T-cells expand in vivo and persist long-term at low levels.