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    • Direct Detection of Flaviviruses Via Gold Nanorod Plasmon Resonance

    openalex（2023）

    Universidade Federal de Ouro Preto | Fundação Oswaldo Cruz | Universidade Federal de Alfenas | Universidade Federal de Minas Gerais

    Cited 0|Views2
    Abstract
    Dengue virus (DENV)1, Zika virus (ZIKV), yellow fever virus, chikungunya virus, and Mayaro virus are medically important arboviruses that cause millions of infections annually in many countries. The clinical diagnosis of these viruses is difficult because of cross-reactivity in some serological tests and requires the development of new diagnostic technologies. In this study, the effectiveness of functionalization methodologies of gold nanorods with specific anti-DENV and anti-flavivirus antibodies was investigated to test the ability of this technology to detect viral particles. Gold nanorods were synthesized, functionalized to 4 mM polyethyleneimine, conjugated with 0.4 μg/mL of monoclonal antibodies against flavivirus, and incubated with different amounts of virus. Viral particles were detected by reading the surface plasmon resonance localized in the UV-Vis scanning spectrometer. Detection of DENV2 and ZIKV was performed in diluted human serum and macerated mosquitoes with a detection limit of 100 PFU/mL for both. Thus, gold nanorods can be used to improve flavivirus diagnostics, making the development of new diagnostic tests faster and more precise than existing techniques and enabling studies of virology vigilance in mosquitoes.
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    Dengue
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    • Pretraining has recently greatly promoted the development of natural language processing (NLP)
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    【要点】：本研究探究了利用功能化的金纳米棒检测黄病毒，如登革病毒和寨卡病毒，提出了一种新的病毒检测方法。
    

    【方法】：通过合成金纳米棒并使用特定抗黄病毒抗体进行功能化，利用表面等离子共振来检测病毒颗粒。
    

    【实验】：实验中合成了金纳米棒，将其与聚乙烯亚胺和针对黄病毒的单克隆抗体结合，然后在不同的病毒量下进行孵育。利用UV-Vis扫描光谱仪检测表面等离子共振来识别病毒颗粒。实验使用稀释的人血清和捣碎的蚊子样本进行了登革病毒2型和寨卡病毒的检测，检测限均为100 PFU/mL。