Spatial reorganization ofEscherichia colichromosome contextualizes triclosan stress-related genetic, epigenetic and transcriptome changes
bioRxiv (Cold Spring Harbor Laboratory)(2023)
摘要
ABSTRACT Changes in the spatial organization of bacterial chromosomes under stress conditions and its biological implications remain poorly understood. We mapped the structural landscape of wild-type and Δ dcm E. coli chromosomes under triclosan stress using Hi-C to identify triclosan-induced chromosomal interaction domains (CIDs). Two CIDs were common to the wild-type and Δ dcm E. coli , including a CID with a common boundary at fabI gene, which encodes the triclosan target. All mutations and structural variants under triclosan stress were observed within or in close proximity to triclosan-induced CIDs. Absence of Dcm methylation impacts both short- and long-range interactions in triclosan stress. Single-base resolution methylome maps reveal hypermethylation of adenines (in wild-type and Δ dcm ) and cytosines (in wild-type) in the two common triclosan-induced CIDs. Furthermore, global gene expression profiling identified enrichment of highly expressed genes within the two common CIDs. Our findings suggest that stress-induced CIDs in E. coli are hotspots for genetic variations and are associated with enhanced transcriptional activity and hypermethylation of Dam/Dcm motifs.
更多查看译文
关键词
of<i>escherichia coli</i>chromosome,genetic,spatial reorganization,stress-related
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要