Supplementary Figure 2 from the E6 Oncoprotein from HPV16 Enhances the Canonical Wnt/β-Catenin Pathway in Skin Epidermis <i>in Vivo</i>

PDF file - 108K, HPV16-E6 enhances canonical Wnt/-catenin signaling pathway in primary human keratinocytes. Wnt/-catenin pathway activation was assessed using the TOPFLASH construct. Human foreskin keratinocytes were co-transfected with, E6, HPV16-E6141-151 (E6PDZ), Dvl2 and B-cat expression vectors. Both, E6 and the mutated form lacking the PDZ-binding domain (E6PDZ), minimally activated the canonical Wnt pathway. However, both of the constructs induced very significant enhancement when they were co-transfected with Dvl2 or -catenin (B-cat), which suggests that this effect was independent of the PDZ-binding domain.