Two Faces of Microscopy

Abstract “Two Faces of Microscopy: Diffraction and Interference” describes the development of principles of microscopy, starting with Joseph Fraunhofer and the principle of diffraction gratings perfected by Henry Rowland for high-resolution spectroscopy. The company of Carl Zeiss advanced microscope technology after enlisting the help of Ernst Abbe who formed a new theory of image formation based on light interference. These ideas were extended by Fritz Zernike in the development of phase-contrast microscopy. The ultimate resolution of microscopes, defined by Abbe and known as the Abbe resolution limit, turned out not to be a fundamental limit, but was surpassed by super-resolution microscopy using concepts of interference microscopy and structured illumination.