Supplementary Figure 2 from CAR T Cells Targeting Podoplanin Reduce Orthotopic Glioblastomas in Mouse Brains

(A) Non-specific killing was also excluded by the observation that EGFRvIII-targeting (3C10)-CAR PBMCs did not display significant lysis to LN319 cells. (B) The FACS-sorted CAR-T cells were subjected to RT-PCR. TNF-α was significantly produced in NZ-1 CAR-T cells that were stimulated by LN319 cells, but IL-2 and CD107a were expressed even in mock CAR-T cells, which suggested that the secretion of IL-2 and CD107a was not specific to PDPN antigen.