Figure S7 from Capping Protein Regulator and Myosin 1 Linker 3 is Required for Tumor Metastasis

Supplementary Figure 7, CARMIL3 regulates β-catenin. Related to Fig. 7. A. qRT-PCR measurement of Ctnnb1 mRNA level in WT and Carmil3 KO 4T1 cells. B. HEK293T cells were transfected with CARMIL3-His or β-catenin-FLAG expressing plasmid. After 48 h, the cells were harvested for protein extraction, followed by immunoprecipitation with FLAG M2 antibody and WB. C. Correlation analysis of CARMIL3 mRNA and β-catenin protein expression with primary tumor samples of TCGA BRCA dataset, n=407, method: spearman.