Aleksic_Supplementary Data from Nuclear IGF1R Interacts with Regulatory Regions of Chromatin to Promote RNA Polymerase II Recruitment and Gene Expression Associated with Advanced Tumor Stage

Supplementary file containing: Supplementary methods Table S1: Primers, probes and siRNAs used in this study. Table S2: Demographics of men treated by radical prostatectomy for prostate cancer. Table S3: IGF-1R scores and clinical parameters in men undergoing radical prostatectomy for localized prostate cancer. Table S4: ChIP-seq: mapped reads. Figure S1: Characterization of IGF-1R expression and subcellular localization in prostate cancer. Figure S2: IGF-1R ChIP-seq in human cancer cells containing nuclear IGF-1R. Figure S3: ChIP-seq identifies IGF-1R binding peaks in promoters of JUN and FAM21 but not CCDN1 or IGF1R. Figure S4: Investigating functional consequences of coincidence of IGF-1R and RNAPol2 recruitment to JUN and FAM21A promoters. Figure S5: Depletion of JUN or FAM21A delays migration towards IGF-1 but has no effect on short-term proliferation. Figure S6: JUN expression does not correlate with total IGF-1R in prostate cancer.