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Decoding the Complexity of On-Target Integration: Characterizing DNA Insertions at the CRISPR-Cas9 Targeted Locus Using Nanopore Sequencing

Juan-Juan Zhao,Xin-Yu Sun, Sai-Ning Tian, Zong-Ze Zhao,Meng-Di Yin,Mei Zhao, Feng Zhang,Si-Ang Li,Zhi-Xue Yang,Wei Wen,Tao Cheng, An Gong,Jian-Ping Zhang,Xiao-Bing Zhang

BMC GENOMICS（2024）

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关键词
CRISPR-Cas9,Gene therapy,DNA integration,Long-range PCR,Nanopore sequencing
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