Polymeric Nanoparticles Loaded with Baccharis Dracunculifolia DC Essential Oil: Preparation, Characterization, and Antibacterial Activity in Milk

Journal of Food Processing and PreservationVolume 44, Issue 9 e14712 ORIGINAL ARTICLE Polymeric nanoparticles loaded with Baccharis dracunculifolia DC essential oil: Preparation, characterization, and antibacterial activity in milk Palmira Penina Raúl Timbe, Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de Alimentos, Universidade Federal do Rio Grande do Sul, Porto Alegre, BrazilSearch for more papers by this authorAmanda de Souza da Motta, orcid.org/0000-0001-8637-3868 Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, BrazilSearch for more papers by this authorHenrique Ataíde Isaía, Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de Alimentos, Universidade Federal do Rio Grande do Sul, Porto Alegre, BrazilSearch for more papers by this authorAdriano Brandelli, Corresponding Author abrand@ufrgs.br orcid.org/0000-0002-9307-6471 Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de Alimentos, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil Correspondence Adriano Brandelli, Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de Alimentos, Universidade Federal do Rio Grande do Sul, ICTA-UFRGS, Av. Bento Gonçalves 9500, Porto Alegre 91501-970, Brazil. Email: abrand@ufrgs.brSearch for more papers by this author Palmira Penina Raúl Timbe, Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de Alimentos, Universidade Federal do Rio Grande do Sul, Porto Alegre, BrazilSearch for more papers by this authorAmanda de Souza da Motta, orcid.org/0000-0001-8637-3868 Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, BrazilSearch for more papers by this authorHenrique Ataíde Isaía, Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de Alimentos, Universidade Federal do Rio Grande do Sul, Porto Alegre, BrazilSearch for more papers by this authorAdriano Brandelli, Corresponding Author abrand@ufrgs.br orcid.org/0000-0002-9307-6471 Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de Alimentos, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil Correspondence Adriano Brandelli, Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de Alimentos, Universidade Federal do Rio Grande do Sul, ICTA-UFRGS, Av. Bento Gonçalves 9500, Porto Alegre 91501-970, Brazil. Email: abrand@ufrgs.brSearch for more papers by this author First published: 01 July 2020 https://doi.org/10.1111/jfpp.14712Citations: 4Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinked InRedditWechat Abstract Eudragit RS100 nanoparticles containing Baccharis dracunculifolia DC essential oil (N-EO) was developed by the nanoprecipitation method. N-EO presented an average diameter of 151.6 nm, zeta potential (ζ) of +51.7 mV, and encapsulation efficiency of 99.4%. The antibacterial activity of N-EO and free essential oil (EO) was evaluated in BHI broth against Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes, and Salmonella Enteritidis. No viable cell counts were detected for all strains tested up to 4 hr incubation with EO. The same effect was achieved at 24 hr by the N-EO, suggesting a controlled release of encapsulated EO. The antimicrobial activity was also tested against L. monocytogenes in milk. Both EO and N-EO lack antibacterial effect in whole milk, while a delayed lag phase was observed in skimmed milk. The N-EO caused no significant lysis of erythrocytes suggesting that it can be safe to eukaryotic cells. Practical applications The results of this work suggest that Eudragit nanoparticles can be suitable carriers for essential oils, maintaining their antimicrobial activities. Nanoparticle encapsulation may be useful for food applications by hiding the intense flavor of EO and providing a controlled release of bioactive components. The reduced activity in milk suggests the interaction between the nanostructures and EO with food components, which merits additional studies. Citing Literature Volume44, Issue9September 2020e14712 RelatedInformation