Supplementary Figures 1-6 from Autocrine Signaling by Wnt-5a Deregulates Chemotaxis of Leukemic Cells and Predicts Clinical Outcome in Chronic Lymphocytic Leukemia

Supplementary Figures 1-6. Supplementary Fig. 1: ROR1 mRNA expression determined by qRT-PCR correlates with the amount of the surface receptor present on primary CLL cells. Supplementary Fig. 2: Expression of ROR1 in the WNT5A-negative and WNT5Apositive(A), and WNT5B-low and WNT5B-high (B) patient cohorts. Supplementary Fig. 3: A-C) Time-to-first treatment (TTFT) of patient groups stratified based on the expression of WNT5 genes was plotted using Kaplan-Meier survival curves. Supplementary Fig. 4: Expression of WNT5B mRNA levels in patients with TP53 mutations. n.s. - not significant (Mann-Whitney test). Supplementary Fig. 5: A) - C) Expression of surface receptors ROR1, CXCR4 and CCR7 was evaluated by vital staining and flow-cytometry (N=14). Supplementary Fig. 6: Freshly isolated CLL cells were treated as indicated and their migration was analysed using the Transwell assays. Supplementary Materials and Methods